at least in the interval for an average offline
Basic data structure trong lập trình
Ngày tải lên :
11/05/2015, 05:53
... coordinate, and then when we got to the top of some wood pieces in the first coordinate - initiate a request from the relevant sections of the tree on the second coordinate int sum_y (int vx, int ... specified value, the specified interval No modification request Required to respond to requests from the following: that means finding the minimum number in the interval that is greater than or equal ... accelerate the work It lies in the fact that when after the call we find the desired leader sets, then remember that at the apex , and all passed the path peaks - this is the leader The easiest...
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Tài liệu Powerful Offline Marketing In The Internet Age ppt
Ngày tải lên :
15/12/2013, 10:15
... postcard that has a secret discount from 10% to whatever on everything they buy in one visit The catch is they have to come into your store to find out the amount of the discount The chance that they ... birthday dinner Generally, nobody is going to come in on their birthday and eat their free dinner by themselves They’re going to bring friends, relatives, you get the idea Here’s a great way to ... give them the contact info I collect from all the stores I JV with in their area Again, you need to include a disclaimer when doing that, but in my tests, the benefit has outweighed the losses In...
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Tài liệu Murray N. Rothbard - History Of Money And Banking In The United States (pdf) pptx
Ngày tải lên :
17/01/2014, 02:20
... culminated in the inflationary recession of 1973–1975 by identifying those actions of the Fed with respect to the money supply that initiated and sustained it.12 The historian, including the ... for or initiated the change that effected a new income distribution However, avoiding such questions leaves the quantitative data themselves ultimately unexplained The reason is that the institutions ... logically inferring from them the laws governing the valuing and pricing of the means or “goods.” As such, economics does not inquire into the individual’s motivations in valuing and choosing specific...
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Tài liệu Engineering Mechanics - StaticsChapter 1Problem 1-1 Represent each of the following combinations of units in the correct SI form using an appropriate prefix: (a) m/ms (b) μkm (c) ks/mg (d) km⋅ μN Units Used: μN = 10−6N kmμkm = 109−6Gs = 10 s pptx
Ngày tải lên :
17/02/2014, 14:20
... determine the orientation θ of the third chain,measured clockwise from the positive x axis, so that the magnitude of force F in this chain is a minimum All forces lie in the x-y plane.What is the ... Engineering Mechanics - Statics Chapter Problem 2-19 The riveted bracket supports two forces Determine the angle θ so that the resultant force is directed along the negative x axis What is the ... means, without permission in writing from the publisher Engineering Mechanics - Statics Chapter Problem 1-4 Represent each of the following combinations of units in the correct SI form: (a) Mg/ms,...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
Ngày tải lên :
18/02/2014, 16:20
... erythromycin, was preincubated for The reaction was initiated by the addition of mm NADPH, and continued for 30 at 37 °C in a shaking water bath The reaction was terminated by the addition of 250 ... lg of protein, and 20 lm resorufin derivatives as a substrate Reactions were initiated by the addition of mm NADPH, and incubation was continued for 30 at 37 °C in a shaking water bath The reactions ... signal, as in the case of CYP1A1 [4]; and (b) PKA- or protein kinase C-mediated phosphorylation of nascent chains either at the N-terminus (Ser128 or Ser129) or the C-terminus, which promotes the mitochondrial...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
Ngày tải lên :
19/02/2014, 06:20
... copper binding protein, and (b) that amyloid fibril formation of this protein is diminished in presence of Cu2+ ions Another interesting finding is that copper binding gets less strong at pH 5, ... maintained at a constant temperature The instrument measured the heat generated or absorbed as the ligand-macromolecule reaction occured A binding isotherm was fitted to the data, expressed in ... tetramerization Possibly, loss of copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable...
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Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc
Ngày tải lên :
19/02/2014, 07:20
... selves which pathway predominates in vivo From the in vitro kinetic data one would predict a preferentially channelling of CH3-4-aminobutyrate to the demethylation pathway, since the kcat ⁄ Km of ... reaction was initiated by the addition of 10 mm substrate (CH3-4-aminobutyrate or 4-aminobutyrate) The oxidation of o-dianisidine was monitored at room temperature by the increase in absorption at 430 ... lacking pAO1 on CH3-4-aminobutyrate, 4-aminobutyrate or methylamine CH3-4-aminobutyrate, 4-aminobutyrate or methylamine (2 gÆL)1) replaced citrate as carbon source in the minimal medium [21] in these...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
Ngày tải lên :
19/02/2014, 13:20
... but rather indicates that the binding process is not associated with a measurable net heat change The finding that binding of UDPNAG to the K22V mutant protein restores the ability to form the ... residues in the loop for binding interactions with the phosphonate group of fosfomycin (or with the phosphate group of phosphoenolpyruvate during normal catalysis) In order to investigate the importance ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins However, two other positively charged amino...
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Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
Ngày tải lên :
19/02/2014, 17:20
... 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the terminal structures of the N-glycans of GAT1, N-glycosylation processing of NNN was inhibited ... after treatment with dMM (Fig 4B) This indicates that the terminal trimming of N-oligosaccharides is not involved in the regulation of plasma membrane trafficking of GAT1, but in the regulation of ... glycine then incubated in 100 mm glycine for 20 The supernatant of the solubilized cells was incubated with 50 lL streptavidin beads (Pierce) at °C for 10 h After extensive washing, all the membrane...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
Ngày tải lên :
20/02/2014, 11:20
... this protein in the matrix We have not detected any significant modification in the amount of this protein in the matrix We can conclude that the decrease in this enzymatic activity is the result ... determination in rat preparations indicated a very low lysosomal contamination (4% of mitochondrial matrix vs total liver homogenate) The activity of ATP-stimulated protease was determined in the presence ... rates were measured polarographically in the presence of 10 mM succinate or mM glutamate plus mM malate State respiration was determined after adding 310 nmoles ADP Glutamate (n ¼ 4) Succinate...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
Ngày tải lên :
07/03/2014, 05:20
... speculate that the arginine residues in ClpA not regulate substrate unfolding directly through interaction with the substrate, but instead coordinate substrate binding ⁄ release via an interaction ... elements in ClpA, one in the N-domain and the other in the pore of the ClpA hexamer In the case of short unstructured peptides or unfolded proteins such as casein, binding to the tyrosine residues in ... substrates, by triggering ‘local’ unfolding of the substrate We speculate that the ATP-dependent global unfolding of some protein substrates is initiated through productive binding to the substrate...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
Ngày tải lên :
07/03/2014, 17:20
... about the amphipathicity of the protein Stefin B aggregates obtained at pH 4.8 or 3.3 insert much more readily into an air–water interface than the native states of stefins A and B obtained at pH ... toxicity A Insertion in monolayers B The ability of stefins and their aggregates to insert at the air–water interface, i.e in the absence of lipids, was determined first, as this may give an indication ... used as the signal hardly changes during the injection and was the same as before the injection during the dissociation phases Weak binding at the micromolar range was observed for stefin B at pH...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
Ngày tải lên :
07/03/2014, 21:20
... than the wildtype V1aR to become activated This implies that arginyl at this locus is a constraining residue which contributes to maintaining the conformational switch of the V1aR in the off-state ... dual role suggests that binding of the agonist releases the Arg46-mediated constraint on the ground state of the receptor, thereby promoting agonistinduced activation Interestingly, Ôdual-roleÕ ... guanidinium could not be co-ordinated within the mutant receptor in the appropriate orientation to recover agonist binding Although Lys46 is an inadequate substitution, charge is nevertheless...
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Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf
Ngày tải lên :
08/03/2014, 08:20
... chain to the nucleotide-binding pocket [3] We describe the engineering, expression and purification of single-cysteine-containing proteins comprising either the C-terminal NBD or the N-terminal ... on p[NH]ppA binding, in agreement with the results obtained here The nonidentical interaction of GTP may be related to another variation in sequence in the vicinity of the ATP-binding pocket, ... N-terminal NBD of P-glycoprotein contains only a single cysteine in the Walker-A motif [43] Thus, MBP-NBD-Nter is a 75-kDa fusion protein containing a single cysteine residue located in the ATP-binding...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
Ngày tải lên :
08/03/2014, 10:20
... University) [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG binding domain, was inserted into NheI-HindIII digested pcDNA3.1, producing the vector, pInsProA pDN42 was ... GalNAc-T1, might be involved in the catalytic function of the enzyme To investigate whether the cysteine residues modified by PCMPS are involved in the binding of UDP-GalNAc, we treated recombinant GalNAc-T1 ... modified by PCMPS may interact with UDPGalNAc, or at least be located in the UDP-GalNAc binding cleft Furthermore, the data suggest that the cysteine residues predominantly interact with a UDP...
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Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot
Ngày tải lên :
16/03/2014, 13:20
... demonstrated that glycosylation of the native site in the S1–S2 linker was required for proper trafficking and stability [43] On the other hand, glycosylation of the native site in the S1–S2 linker ... conformation is flexible [42] The Kv3.1 results demonstrated that both glycosylation sites in the S1–S2 linker were utilized, indicating that both of these regions can accommodate a conformation ... results indicate that the occupancy of the N-glycosylation sites is a determining factor for the voltage-dependent activation kinetics of Kv3.1 A recent report on Kv1.1 indicated that N-glycosylation...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
Ngày tải lên :
16/03/2014, 18:20
... complexes (B) The binding of in vitro translated ERM is eliminated by mutations in Ets motifs The binding of in vitro translated ERM over the PS1 promoter was assayed by EMSAs including the 32P-labeled ... motifs in the )119/+178 region of the PS1 promoter are indicated The positions relative to the +1 site of point mutations (X) eliminating Ets or Sp1 consensus in each motif are indicated on the ... ERM being the most related [35] PEA3 members all contain the highly conserved ETS domain ERM contains two DNA binding inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory...
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Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
Ngày tải lên :
16/03/2014, 23:20
... have investigated the effects of mutation of two paradigmatic residues involved in the dislocation of BS-RNase N-terminal arms, i.e Pro19 and Leu28, located in the hinge peptide and in the intersubunit ... comparable with that of parent mBS, indicating that a native conformation is present A further indication of the similarity of their global fold to that of the parent protein is provided by the 1D H-NMR ... the recombinant sample [11] The kinetics of the N-terminal arms swapping in the P19A-BSRNase is comparable to that of BS-RNase (Fig 5B) Interestingly, the amount of the MxM form at the plateau as...
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